World Health Organization: Multiple-Tube Method for Thermotolerant (Faecal) Coliforms

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The safe quality of water supplied to communities is an important consideration in the protection of human health and well-being, it is not the only factor that affects consumers.

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When water samples are collected for analysis, care should be taken to ensure that there is no external contamination of the samples. Unless valid samples are collected, the results of the subsequent analysis may be misleading. The World Health Organization: Multiple-Tube Method for Thermotolerant (Faecal) Coliforms mobile app provides a detailed list for the Multiple-Tube Method for Thermotolerant (Faecal) Coliforms.

In the multiple-tube method, a series of tubes containing a suitable selective broth culture medium is inoculated with test portions of a water sample. After a specified incubation time at a given temperature, each tube showing gas formation is regarded as “presumptive positive” since the gas indicates the possible presence of coliforms.

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